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Luke

Luke Castellan, who used the venom to poison Thalia's Tree.

Elder Python Venom is a venom derived from the Elder Python monster. According to Chiron, the Camp Half-Blood activities director, the poison originates from the depths of Tartarus.

Percy Jackson and the Olympians

The Sea of Monsters

Luke Castellan uses the venom it to poison Thalia's tree, thus forcing Camp Half-Blood to issue a quest to retrieve the Golden Fleece (the only thing that can heal it) from Polyphemus' Island. The Golden Fleece not only heals the tree of its poison, but restores Thalia Grace to life as well, giving Kronos another chance to control the Great Prophecy.

Items
Personal Weapons: Riptide | Annabeth's Knife | Backbiter | Frank's Spear | Hazel's Spatha | Katoptris | Nico's Sword | Thalia's Spear | Aegis | Maimer | Kronos' Scythe | Ivlivs | Master Bolt | Poseidon's Trident | Sword of Hades | Reyna's Spear | Hades' Staff | Juno's Gladius | Annabeth's Sword | Sumarbrander | Mjølnir | Thor's Staff | Khopesh | Mallory's Serrated Knife | Meg's Twin Imperial Gold siccea blades
Magical Items: Annabeth's Yankees Cap | Helm of Darkness | Keys of Hades | Flying Chariot | Golden Apple | Greek Fire | Hermes' Multivitamins | Leo's Magical Toolbelt | Nectar and Ambrosia | Pandora's Pithos | Winged Shoes | The Golden Fleece | Stygian Ice Whistle | Arrow of Dodona | Serapis' Staff | Magic 8 Ball
Spoils of War: The Minotaur's Horn | Medusa's Head | Kampê's Scimitars | Nemean Lion's Pelt | Gorgon Blood | Cornucopia | Lydian Drakon Hide | Phineas' Robe and Slippers
Items: Camp Necklace | Chameleon Armor | Daedalus' Laptop | Golden drachma | Denarius | Mark of Athena | The Pax | Video Shield | Wristwatch Shield | Golden Mango | Sibylline Books
Blessed Metals: Celestial Bronze | Imperial Gold | Stygian Iron | Bone Steel